Analysis of Effect of Low Temperature on Seed Germination of Aleurites fordii in Tung Oil by Plant Respirometer

Low temperature stress is one of the main external environmental factors that affect plant life activities. Under low temperature conditions, the plant cell structure and physiological and biochemical functions have produced a series of changes. First of all, the hypothesis of "film damage" of chilling injury of plants was proposed. Many studies have proved that low temperature can obviously cause the destruction of plant cell membrane system. However, there is no unified opinion on the mechanism of membrane system destruction. At present, there is very little research on the damage of tung oil to low temperature. This experiment is to use tung tree seeds and seedlings as materials, through artificial low-temperature treatment combined with biochemical analysis. Effects of Low Temperature on Seed Germination and Seedling Growth of Aleurites tabulaeformis and Its Influencing Mechanism The plant respiration analyzer is capable of performing an effective measurement and analysis of its respiration.

Low temperatures caused a decrease in ascorbic acid content in seeds and seedlings of Aleurites fordii. Exogenous ascorbic acid treatment can prevent the increase of K+ infiltration rate and malondialdehyde content of Aleurites fordii seedlings, inhibit the decrease of the rate of TTC reduction in leaf cells, and increase the heat-cooling of seedlings. Pretreatment of tung oil seedlings with 10 mm Vc for 2 days not only increased the ascorbic acid content but also increased the activity of superoxide dismutase and catalase at low temperatures, thereby increasing the ability to scavenge reactive oxygen species. The cold resistance of plants is related to the reduction of reactive oxygen species and the production of reactive oxygen species.

The determination of plant resorption by the plant respiration tester found that the degree of damage caused by low-temperature stress in the plant tissues depends on the severity of the external hypothermia, the duration of action, and the resistance to cold tissue. The balance between the production and elimination of reactive oxygen species in plants plays an important role in the cold resistance of plants. Under low temperature stress, the balance in plants is destroyed, resulting in increased production of reactive oxygen species and reduced removal. Active oxygen accumulates in the body and exceeds the damage threshold value. On the one hand, it causes the destruction of biological functional molecules such as chlorophyll, proteins and nucleic acids; on the other hand, it causes the increase of membrane lipid peroxidation and the enhancement of membrane lipid decarboxylation, which destroys the integrity of the membrane. Loss of differential permeability, leakage of electrolytes and certain small-molecule organics, and disruption of the exchange of cellular materials, causing a series of physiological and metabolic disorders, causing plant death in severe cases.

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